Major Highlights at Research Front
Faculty and students of DSATM community are involved in path-breaking research activities. DSATM follows the key ethical principles that should underlie the research endeavor. Researchers here take on real time problems, think innovatively and bring in novel solutions, to advance knowledge and make meaningful contribution to the society.
“Study of formation of DNA adducts with Phenanthrene and Benzo(a) Pyrene and its Characterization “.
Phenanthrene and Benzo(a) pyrene like Polycyclic aromatic Hydrocarbons ( PAHs) are usually present in cooked foods, cigarettes smoke .They also formed during frying & grilling of meat and fish. These may be possibly involved in carcinogenesis in human beings.
This prompted us to study the identification of PAHs particularly Phenanthrene adducts with DNA in order to understand the potential genotoxic effects.
Kroum Alexandrov et al. Cancer Res 2006;66:1193
This work is divided into three phases.
In the first phase, the selected PAHs and HPLC instrument was purchased .All chemicals and reagents of analytical grade were purchased commercially and used without further purification unless otherwise noted.Phenanthrene and Benzo(a) pyrene was purchased from Sigma company and Calf thymus from SRL company.The HPLC system was from Agilent Technologies
The Solutions of CT-DNA in 5 mM Tris–HCl/50 mM NaCl [pH = 7.2, Tris = Tris(hydroxymethyl)aminomethane] gave a ratio of UV absorbance at 260 and 280 nm of 1.8–1.9:1, indicating that the DNA was sufficiently free of protein. The adducts of DNA and Phananthrene were prepared in ration of 2;1 and adduct formation was verified on gel electrophoresis,The chromatographic separation of Phenanthrene-DNA adducts are under process. The analytes will be identified using FT-MS in further phases
The Free Scale Cup Kit sanctioned by shenoy Systems to CSE department.
The traffic management in today’s world has been a major concern for most people. The safety on the road has been a big challenge in today’s world. Disobeying Traffic rules is one of the major cause for road accidents. The current traffic rule governance is achieved by a trivial method by displaying the rules as symbols and signs on the roads.In this work an intelligent car system incorporates a microcontroller such as Freescale FRDM KL125Z chip to takes various input parameters from its environment through a camera
These parameters are processed to perform the requisite controlling of the car. The system is aware about traffic rules on various speed regulation, one-way regulation and curve assist etc. The proposed system showed promising results to ensure practical road safety.